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Assessment of Antioxidative Activity of Lipid- and Water-Soluble Vitamins in Human Whole Blood. Comparative Analysis Between a Biological Test and Chemical Methods

Lesgards, Jean-Francois, Lehucher-Michel, Marie-Pascale, Vidal, Nicolas, Stocker, Pierre
International journal for vitamin and nutrition research 2005 v.75 no.1 pp. 11-18
humans, blood, cultured cells, antioxidant activity, fat soluble vitamins, water-soluble vitamins, erythrocytes, blood plasma, free radicals, chemiluminescence, hemolysis, blood gases
Assessment of the antioxidant activity of vitamins and other compounds is of interest in the understanding of their in vivo effects. In this study, we have investigated the activity of several lipid and water-soluble vitamins in human whole blood. Measurements were carried out using a biological test that enables the evaluation of both red blood cells and plasma resistance against free radical activity induced by 2,2'-azobis (2-amidinopropane)hydrochloride (AAPH). Antioxidant activity of vitamins has been determined by using the biological test versus chemical methods (chemiluminescence, DMPD radical). We have observed strong antioxidant potentials for vitamins B<sub>6</sub> and B<sub>9</sub> with biological tests, but not with chemical methods. At 10 μM, the vitamin B<sub>9</sub> efficiency in inhibiting radical-induced red blood cell hemolysis was almost three times higher than vitamin C efficiency and two times higher than α-tocopherol efficiency. Antioxidant activity was not observed for vitamins B<sub>1</sub> or B<sub>2</sub>, nor for retinol. The weak activity of β-carotene still remains to be investigated particularly in relation to oxygen pressure. Our study demonstrated that the biological test is more useful than the chemical methods employed in this instance, for the evaluation of antioxidant capacity of lipophilic and putatively biologically active compounds.