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Ctf18 is required for homologous recombination-mediated double-strand break repair

Ogiwara, Hideaki, Ohuchi, Takashi, Ui, Ayako, Tada, Shusuke, Enomoto, Takemi, Seki, Masayuki
Nucleic acids research 2007 v.35 no.15 pp. 4989-5000
DNA repair, chromatids, chromatin, chromosome segregation, cohesion, homologous recombination, mutagens, mutants, nucleic acids, yeasts
The efficient repair of double-strand breaks (DSBs) is crucial in maintaining genomic integrity. Sister chromatid cohesion is important for not only faithful chromosome segregation but also for proper DSB repair. During DSB repair, the Smc1-Smc3 cohesin complex is loaded onto chromatin around the DSB to support recombination-mediated DSB repair. In this study, we investigated whether Ctf18, a factor implicated in the establishment of sister chromatid cohesion, is involved in DSB repair in budding yeast. Ctf18 was recruited to HO-endonuclease induced DSB sites in an Mre11-dependent manner and to damaged chromatin in G₂/M phase-arrested cells. The ctf18 mutant cells showed high sensitivity to DSB-inducible genotoxic agents and defects in DSB repair, as well as defects in damage-induced recombination between sister chromatids and between homologous chromosomes. These results suggest that Ctf18 is involved in damage-induced homologous recombination.