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Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli

Author:
Di Lorenzo, Mirella, Hidalgo, Aurelio, Haas, Michael, Bornscheuer, Uwe T.
Source:
Applied and environmental microbiology 2005 v.71 no.12 pp. 8974
ISSN:
0099-2240
Subject:
Rhizopus oryzae, triacylglycerol lipase, complementary DNA, molecular cloning, gene transfer, gene expression, Escherichia coli, protein synthesis, recombinant proteins, enzyme activity
Abstract:
To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.
Agid:
19976
Handle:
10113/19976