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Comparison of aerobic denitrification under high oxygen atmosphere by Thiosphaera pantotropha ATCC 35512 and Pseudomonas stutzeri SU2 newly isolated from the activated sludge of a piggery wastewater treatment system

Su, J.J., Liu, B.Y., Liu, C.Y.
Journal of applied microbiology 2001 v.90 no.3 pp. 457-462
Paracoccus pantotrophus, Pseudomonas stutzeri, activated sludge, batch systems, blood serum, bottles, culture media, denitrification, nitrates, nitrites, nitrogen, oxygen, wastewater, wastewater treatment
Aims: This study compares the ability of Thiosphaera pantotropha ATCC 35512 and the newly isolated Pseudomonas stutzeri SU2 to perform aerobic denitrification. Methods and Results: Nitrate-supplemented basal medium in airtight crimp-sealed serum bottles containing an atmosphere of 92% oxygen was inoculated with Ps. stutzeri SU2 or T. pantotropha and incubated at 30 degrees C. During the 92-h incubation period, aerobic denitrification by Ps. stutzeri SU2 (NO3(-)-N removal 99.24%) was more efficient than that by T. pantotropha (NO3(-)-N removal 27.29%). Conclusions: Pseudomonas stutzeri SU2, which was isolated from the activated sludge of a sequencing batch reactor treating piggery wastewater, rapidly reduced the nitrate to nitrogen gas without nitrite accumulation. The nitrate removal rate of SU2 was 0.032 mmol NO3(-)-N g cell-1 h-1 after 44 h incubation. Significance and Impact of the Study: Pseudomonas stutzeri SU2 can be used in a full-scale sequencing batch system for efficient in situ aerobic nitrate removal from piggery wastewater.