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Mutagenesis of Clostridium butyricum

Author:
Carrasco, Alicia, Soto, C.
Source:
Journal of applied microbiology 1987 v.63 no.6 pp. 539-543
ISSN:
1364-5072
Subject:
Clostridium butyricum, antibiotic resistance, irradiation, methane, microbiology, mutagenesis, mutagenicity, mutants
Abstract:
Mutagenesis of Clostridium butyricum ATCC 19398 was best accomplished by u. v. irradiation (254 nm) and methyl methane sulphonate. Nitrosoguanidine was only slightly mutagenic for this organism. Procedures are described which yielded a variety of auxotrophic and antibioticā€resistant mutant strains.