Jump to Main Content
A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome
- Sultan, Marc, Schulz, Marcel H., Richard, Hugues, Magen, Alon, Klingenhoff, Andreas, Scherf, Matthias, Seifert, Martin, Borodina, Tatjana, Soldatov, Aleksey, Parkhomchuk, Dmitri, Schmidt, Dominic, O'Keeffe, Sean, Haas, Stefan, Vingron, Martin, Lehrach, Hans, Yaspo, Marie-Laure
- Science 2008 v.321 no.5891 pp. 956-960
- alternative splicing, humans, microarray technology, high-throughput nucleotide sequencing, surveys, transcriptome, kidneys, exons
- The functional complexity of the human transcriptome is not yet fully elucidated. We report a high-throughput sequence of the human transcriptome from a human embryonic kidney and a B cell line. We used shotgun sequencing of transcripts to generate randomly distributed reads. Of these, 50% mapped to unique genomic locations, of which 80% corresponded to known exons. We found that 66% of the polyadenylated transcriptome mapped to known genes and 34% to nonannotated genomic regions. On the basis of known transcripts, RNA-Seq can detect 25% more genes than can microarrays. A global survey of messenger RNA splicing events identified 94,241 splice junctions (4096 of which were previously unidentified) and showed that exon skipping is the most prevalent form of alternative splicing.