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Preparative Isolation and Purification of Altertoxin I from an Alternaria sp. by HSCCC
- Hu, Dejun, Liu, Miao, Xia, Xing, Chen, Daijie, Zhao, Fengsheng, Ge, Mei
- Chromatographia 2008 v.67 no.11-12 pp. 863-867
- Alternaria, acetone, countercurrent chromatography, ethyl acetate, fermentation, food pathogens, fruits, grains, mutagenicity, mycotoxins, nuclear magnetic resonance spectroscopy, solvents, toxicology
- Altertoxin I (ATX I) is one of the common mycotoxins produced by genus Alternaria which is a common food pathogen of fruits and grains. To prepare enough quantity of pure ATX I for further research of mutagenicity and toxicology tests, a novel method using preparative high-speed counter-current chromatography (HSCCC) was developed. The ethyl acetate crude extracts of the acetone washes obtained after fermentation of Alternaria sp. was separated using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (2:5:5:6, v/v). Collected fractions were analyzed by LC and identified by EI-MS and NMR analysis. The technique can isolate mg levels of the target compound per run.