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Peptide substrate for caspase-3 with 2-aminoacridone as reporting group
- Lozanov, Valentin, Ivanov, Ivaylo P., Benkova, Bistra, Mitev, Vanio
- Amino acids 2009 v.36 no.3 pp. 581-586
- caspase-3, enzyme activity, fluorescence, peptides
- Synthesis and properties of a new fluorescent/fluorogenic substrate Ac-DEVD-AMAC for caspase-3 are reported. The substrate is obtained by conventional Fmoc-based solid phase peptide synthesis and its properties are investigated with regard to fluorescence, sensitivity, applicability and kinetic constants. A non-traditional approach to assay the proteases activity using 2-aminoacridone labeled peptides is proposed. This approach utilizes the decrease of fluorescence intensity of a sample as a measure for the enzyme activity.