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Growth factorâinduced promoter activation of murine phospholipase C Î´4 gene
- Fukami, Kiyoko, Takenaka, Kei, Nagano, Kohji, Takenawa, Tadaomi
- European journal of biochemistry 2000 v.267 no.1 pp. 28-36
- DNA, TATA box, blood serum, bradykinin, calcium, gels, genes, growth factors, isozymes, luciferase, messenger RNA, mice, neoplasm cells, phospholipase C, sequence analysis
- Phospholipase C Î´4 (PLCÎ´4) is one of the deltaâtype PLC isozymes, the expression of which is induced in nuclei by treatment with serum and also in some cancer cells. We isolated and analyzed a promoter region of the murine PLCÎ´4 gene. DNA sequence analysis showed that this region is GCârich and has no TATA box, and the region from â143 to â127 was found, by luciferase activity and gel mobilityâshift assay, to be essential for transcription of PLCÎ´4. We also found that the promoter activity of PLCÎ´4 was stimulated by treatment with growth factors such as bradykinin, lysophosphatidic acid, and Ca2+ ionophore in addition to serum. In parallel, we detected PLCÎ´4 mRNA induction and an increase in complex formation of the promoter region and nuclear protein from HeLa cells on stimulation with these growth factors. Finally, we found that trapping the growth factorâinduced cytoplasmic Ca2+âinhibited activation of the promoter activity and protein induction in nuclei. These results show that PLCÎ´4 may have an important role in nuclei in response to growth factors, and its expression may be partially regulated by an increase in cytoplasmic Ca2+.