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Interspecific hybridization of Cucumis anguria and C. zeyheri via embryo-rescue
- Skálová, D., Dziechciarková, M., Lebeda, A., Křístková, E., Navrátilová, B.
- Biologia plantarum 2008 v.52 no.4 pp. 775-778
- Cucumis anguria, ascorbic acid, coconut water, embryo (plant), embryogenesis, fruits, germination, hybrids, interspecific hybridization, leucyl aminopeptidase, loci, parents, plant development, pollination
- Embryo-rescue was used to facilitate interspecific hybridization of Cucumis anguria L. and C. zeyheri Sond. Embryos were excised from developing fruits at one week intervals for six weeks after hand pollination. Medium containing coconut water was the most suitable for initial germination, and a medium with ascorbic acid was the best for embryo development and plant recovery. Viable plants were obtained from embryos and these plants showed morphological characteristics different from both parents. The analysis of the leucine aminopeptidase (LAP) locus revealed three hybrid types, H1.1, H1.2 and H2.