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Isolation and functional characterization of HvDREB1--a gene encoding a dehydration-responsive element binding protein in Hordeum vulgare

Xu, Zhao-Shi, Ni, Zhi-Yong, Li, Zhi-Yong, Li, Lian-Cheng, Chen, Ming, Gao, Dong-Yao, Yu, Xiu-Dao, Liu, Pei, Ma, You-Zhi
Journal of plant research 2009 v.122 no.1 pp. 121-130
Arabidopsis thaliana, Hordeum vulgare, abscisic acid, barley, binding proteins, drought, gene overexpression, leaves, mutants, reporter genes, salt stress, salt tolerance, seedlings, transcription factors, transcriptional activation, yeasts
A gene encoding Hordeum vulgare dehydration-responsive element binding protein 1 (HvDREB1), a member of the A-2 subgroup of the DREB subfamily, was isolated from barley seedlings. A subcellular localization assay revealed accumulation of HvDREB1 protein in the nucleus. As a trans-acting factor, HvDREB1 was able to bind to DRE/CRT elements and transactivate reporter gene expression in yeast cells. A study of various deletion mutants of HvDREB1 proteins indicated that the transactivation activity was localized to the N-terminal region. Expression of the HvDREB1 gene in barley leaves was significantly induced by salt, drought, and low-temperature. In contrast to most A-2 subgroup members in Arabidopsis thaliana, HvDREB1 also responded to exogenous ABA. Overexpression of HvDREB1 activated a downstream gene, RD29A, under normal growth conditions and led to increased tolerance to salt stress in Arabidopsis plants. These results suggest that HvDREB1 produces a DRE-/CRT-binding transcription factor that may have an important role in improving salt tolerance in plants.