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The physiology of Clostridium sporogenes NCIB 8053 growing in defined media
- Lovitt, R. W., Kell, D. B., Morris, J. G.
- Journal of applied microbiology 1987 v.62 no.1 pp. 81-92
- Clostridium sporogenes, acetates, energy, enzymes, ethanol, glucose, glycolysis, microbiology, proline
- The physiology of Clostridium sporogenes was investigated in defined, minimal media. In batch culture, the major end products of glucose dissimilation were acetate, ethanol and formate. When Lâproline was present as an electron acceptor, acetate production was strongly enhanced at the expense of ethanol. As judged by assay of the relevant enzymes, glucose was metabolized via the EmbdenâMeyerhofâParnas pathway. The growth energetics of Cl. sporogenes were investigated in glucoseâ or Lâvalineâlimited chemostat cultures. In the former case, the addition of Lâproline to the medium caused a significant increase in the molar growth yield (as calculated by extrapolation to infinite dilution rate). This finding adds weight to the view that the reduction of Lâproline by Cl. sporogenes is coupled to the conservation of free energy.