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Fast DNA-based identification of the black truffle Tuber melanosporum with direct PCR and species-specific primers

Author:
Bonito, Gregory
Source:
FEMS microbiology letters 2009 v.301 no.2 pp. 171-175
ISSN:
0378-1097
Subject:
Tuber melanosporum, mushrooms, phylogeny, polymerase chain reaction, ribosomal DNA, truffles
Abstract:
A quick and sensitive DNA-based assay for identifying the black truffle species Tuber melanosporum is presented. Both direct PCR and species-specific primers are used to amplify a phylogenetically informative region of T. melanosporum rDNA. This method was successfully used to screen fresh and frozen T. melanosporum fruitbodies, and could be modified for the molecular detection of other truffle and mushroom species.
Agid:
2209425