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Extensive, Recent Intron Gains in Daphnia Populations

Author:
Li, Wenli, Tucker, Abraham E., Sung, Way, Thomas, W. Kelley, Lynch, Michael
Source:
Science 2009 v.326 no.5957 pp. 1260-1262
ISSN:
0036-8075
Subject:
Daphnia pulex, alleles, exons, genetic drift, introns, loci, organisms, phylogeny
Abstract:
Rates and mechanisms of intron gain and loss have traditionally been inferred from alignments of highly conserved genes sampled from phylogenetically distant taxa. We report a population-genomic approach that detected 24 discordant intron/exon boundaries between the whole-genome sequences of two Daphnia pulex isolates. Sequencing of presence/absence loci across a collection of D. pulex isolates and outgroup Daphnia species shows that most polymorphisms are a consequence of recent gains, with parallel gains often occurring at the same locations in independent allelic lineages. More than half of the recent gains are associated with short sequence repeats, suggesting an origin via repair of staggered double-strand breaks. By comparing the allele-frequency spectrum of intron-gain alleles with that for derived single-base substitutions, we also provide evidence that newly arisen introns are intrinsically deleterious and tend to accumulate in population-genetic settings where random genetic drift is a relatively strong force.
Agid:
2211675