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Gastroprotective mechanisms of the chloroform and ethyl acetate phases of Praxelis clematidea (Griseb.) R.M.King & H.Robinson (Asteraceae)

Author:
Falcão, Heloina de Sousa, Maia, Gabriela Lemos de Azevedo, Bonamin, Flávia, Kushima, Hélio, Moraes, Thiago Mello, Hiruma Lima, Clélia Akiko, Takayama, Christiane, Ferreira, Anderson Luiz, Souza Brito, Alba Regina Monteiro, Agra, Maria de Fátima, Barbosa Filho, José Maria, Batista, Leônia Maria
Source:
Natural medicines 2013 v.67 no.3 pp. 480-491
ISSN:
1340-3443
Subject:
Asteraceae, aerial parts, chloroform, ethanol, ethyl acetate, glutathione, glutathione peroxidase, glutathione-disulfide reductase, indigenous species, lipid peroxidation, malondialdehyde, models, mucus, myeloperoxidase, nitric oxide, nonsteroidal anti-inflammatory agents, prostaglandins, pylorus, secretion, stomach ulcers, superoxide dismutase, South America
Abstract:
Flavonoid-rich Praxelis clematidea (Griseb.) R.M.King & H.Robinson (Asteraceae) is a native plant of South America. This study evaluates the gastroprotective activity and possible mechanisms for both the chloroform (CHCl₃P) and ethyl acetate phases (AcOEtP) obtained from aerial parts of the plant. The activity was investigated using acute models of gastric ulcer. Gastric secretion biochemical parameters were determined after pylorus ligature. The participation of cytoprotective factors such as mucus, nitric oxide (NO), sulfhydryl (SH) groups, prostaglandin E₂(PGE₂), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), reduction of lipid peroxidation (malondialdehyde level), and polymorphonuclear infiltration (myeloperoxidase activity), was also investigated. CHCl₃P (125, 250, and 500 mg/kg) and AcOEtP (62.5, 125, and 250 mg/kg) showed significant gastroprotective activity, reducing the ulcerative index by 75, 83, 88 % and 66, 66, 81 % for ethanol; 67, 67, 56 % and 56, 53, 58 % for a non-steroidal anti-inflammatory drug (NSAID); and 74, 58, 59 % and 64, 65, 61 % for stress-induced gastric ulcer, respectively. CHCl₃P (125 mg/kg) and AcOEtP (62.5 mg/kg) significantly reduced the ulcerative area by 78 and 83 %, respectively, for the ischemia–reperfusion model. They also did not alter the biochemical parameters of gastric secretion, the GSH level or the activities of SOD, GPx or GR. They increased the quantity of gastric mucus, not dependent on NO, yet dependent on SH groups, and maintained PGE₂levels. The P. clematidea phases demonstrated gastroprotective activity related to cytoprotective factors.
Agid:
222334