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CE-ESI-TOF/MS for human growth hormone analysis
- Staub, Aline, Giraud, Sylvain, Saugy, Martial, Rudaz, Serge, Veuthey, Jean-Luc, Schappler, Julie
- Electrophoresis 2010 v.31 no.2 pp. 388-395
- electrophoresis, experimental design, human growth, proteins, somatotropin
- CE is a powerful analytical tool used to separate intact biomolecules such as proteins. The coupling of CE with TOF/MS produces a very promising method that can be used to detect and identify proteins in different matrices. This paper describes an efficient, rapid, and simple CE-ESI-TOF/MS procedure for the analysis of endogenous human growth hormone and recombinant human growth hormone without sample preparation. Operational factors were optimized using an experimental design, and the method was successfully applied to distinguish human growth hormone and recombinant human growth hormone in unknown samples.