Main content area

Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells

Choi, Jiwon, Kang, Hyun Ju, Kim, Sung Zoo, Kwon, Tae Oh, Jeong, Seung-Il, Jang, Seon Il
Archives of pharmacal research 2013 v.36 no.7 pp. 912-917
Morus alba, active oxygen species, antioxidant activity, antioxidants, dose response, erythrocytes, glutathione, hemolysis, high performance liquid chromatography, humans, kaempferol, leaves, medicinal plants, methanol, mulberries, oxidation, protective effect, quercetin
We evaluated the antioxidant properties of mulberry leaves extract (MLE) and flavonoids isolated from MLE. MLE was prepared by extraction with methanol. Flavonoids were analyzed by high-performance liquid chromatography. Oxidative hemolysis of normal human red blood cells (RBCs) was induced by the aqueous peroxyl radical [2,2′-Azobis (2-amidinopropane) dihydrochloride, AAPH]. MLE contained three flavonoids in the order quercetin (QC) > kaempferol (KF) > astragalin (AG). Oxidative hemolysis of RBCs induced by AAPH was suppressed by MLE, AG, KF, and QC in a time- and dose-dependent manner. MLE and these three flavonoids prevented the depletion of cystosolic antioxidant glutathione (GSH) in RBCs. AG had the greatest protective effect against AAPH-induced oxidative hemolysis and GSH depletion in RBCs.