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Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells

Author:
Choi, Jiwon, Kang, Hyun Ju, Kim, Sung Zoo, Kwon, Tae Oh, Jeong, Seung-Il, Jang, Seon Il
Source:
Archives of pharmacal research 2013 v.36 no.7 pp. 912-917
ISSN:
0253-6269
Subject:
Morus alba, active oxygen species, antioxidant activity, antioxidants, dose response, erythrocytes, glutathione, hemolysis, high performance liquid chromatography, humans, kaempferol, leaves, medicinal plants, methanol, mulberries, oxidation, protective effect, quercetin
Abstract:
We evaluated the antioxidant properties of mulberry leaves extract (MLE) and flavonoids isolated from MLE. MLE was prepared by extraction with methanol. Flavonoids were analyzed by high-performance liquid chromatography. Oxidative hemolysis of normal human red blood cells (RBCs) was induced by the aqueous peroxyl radical [2,2′-Azobis (2-amidinopropane) dihydrochloride, AAPH]. MLE contained three flavonoids in the order quercetin (QC) > kaempferol (KF) > astragalin (AG). Oxidative hemolysis of RBCs induced by AAPH was suppressed by MLE, AG, KF, and QC in a time- and dose-dependent manner. MLE and these three flavonoids prevented the depletion of cystosolic antioxidant glutathione (GSH) in RBCs. AG had the greatest protective effect against AAPH-induced oxidative hemolysis and GSH depletion in RBCs.
Agid:
224406