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Comparative studies of skeletal muscle proteome and transcriptome profilings between pig breeds

Kim, Nam-Kuk, Park, Hye-Ran, Lee, Hwi-Cheul, Yoon, Duhak, Son, Eun-Suk, Kim, Yeun-Song, Kim, Se-Ra, Kim, Oun-Hyun, Lee, Chang-Soo
Mammalian genome 2010 v.21 no.5-6 pp. 307-319
NADH dehydrogenase (ubiquinone), acyl-CoA dehydrogenase, body weight, databases, energy, gene expression, genes, glycerol-3-phosphate dehydrogenase, growth performance, heat shock proteins, intramuscular fat, landraces, lipid metabolism, meat quality, messenger RNA, microarray technology, muscles, proteome, skeletal muscle, succinate dehydrogenase, swine, swine breeds, transcriptome, two-dimensional gel electrophoresis
Two genetically different pig breeds, the Korean native pig (KNP) and the Western meat-producing Landrace, show breed-specific traits in stress responsiveness (stress hormone levels), growth performance (live weight), and meat quality (intramuscular fat content). We analyzed expression levels within the proteome and transcriptome of the longissimus muscles of both breeds using two-dimensional electrophoresis (2-DE) and microarray analysis. We constructed a porcine proteome database focused mainly on mitochondrial proteins. In total, 101 proteins were identified, of which approximately 60% were metabolic enzymes and mitochondrial proteins. We screened several proteins and genes related to stress and metabolism in skeletal muscles using comparative analysis. In particular, three stress-related genes (heat shock protein β-1, stress-70 protein, and heat shock 70 kDa protein) were more highly expressed in the Landrace than in the KNP breed. Six metabolism-related genes (peroxisome proliferative activated receptor α, short-chain acyl-CoA dehydrogenase, succinate dehydrogenase, NADH-ubiquinone oxidoreductase, glycerol-3-phosphate dehydrogenase, and sterol regulatory element binding protein-1c), all of which are involved in energy and lipid metabolism, were more highly expressed at the protein or mRNA level in the KNP breed. These data may reflect the breed dependence of traits such as stress responsiveness, growth performance, and meat quality.