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pCEC coupling with ESI-MS for the analysis of β₂-agonists and narcotics using a poly-(1-hexadecene-co-TMPTMA) monolithic column

Cheng, Jintian, Zhang, Lan, Lu, Qiaomei, Lu, Minghua, Chen, Zongbao, Chen, Guonan
Electrophoresis 2010 v.31 no.12 pp. 1991-1997
ammonium acetate, beta-adrenergic agonists, electrophoresis, electrospray ionization mass spectrometry, narcotics, pH, polymerization, rapid methods, urine
A pressure-assisted CEC with ESI-MS based on poly(1-hexadecene-co-trimethylolpropane trimethacrylate) monolithic column for rapid analysis of two β₂-agonists and three narcotics was established in this article. After the organic polymer-based monolithic column was prepared by an in-situ polymerization procedure, a systematic investigation of the pressure-assisted CEC separation and ESI-MS detection parameters was performed. Baseline separation of the studied analytes could be obtained using the solution containing 75% ACN v/v and 20 mmol/L ammonium acetate with pH 8.0 as running buffer, when applying separation voltage of 20 kV and assisted pressure of 5 bar. Under the optimized conditions, two β₂-agonists and three narcotics could be completely resolved and accurately determined within 15 min. Finally, the proposed method was successfully used for real urine samples detection.