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TaNF-YC11, one of the light-upregulated NF-YC members in Triticum aestivum, is co-regulated with photosynthesis-related genes

Author:
Stephenson, Troy J., McIntyre, C. Lynne, Collet, Christopher, Xue, Gang-Ping
Source:
Functional & integrative genomics 2010 v.10 no.2 pp. 265-276
ISSN:
1438-793X
Subject:
Calvin cycle, Triticum aestivum, binding sites, data collection, energy, enzymes, gene expression regulation, genes, leaves, reverse transcriptase polymerase chain reaction, seedlings, sequence analysis, solar energy, thylakoids, transcription factors, wheat
Abstract:
Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor complex. Each of the NF-Y subunits (NF-YA, NF-YB and NF-YC) in plants is encoded by multiple genes. Quantitative RT-PCR analysis revealed that five wheat NF-YC members (TaNF-YC5, 8, 9, 11 and 12) were upregulated by light in both the leaf and seedling shoot. Co-expression analysis of Affymetrix wheat genome array datasets revealed that transcript levels of a large number of genes were consistently correlated with those of the TaNF-YC11 and TaNF-YC8 genes in three to four separate Affymetrix array datasets. TaNF-YC11-correlated transcripts were significantly enriched with the Gene Ontology term photosynthesis. Sequence analysis in the promoters of TaNF-YC11-correlated genes revealed the presence of putative NF-Y complex binding sites (CCAAT motifs). Quantitative RT-PCR analysis of a subset of potential TaNF-YC11 target genes showed that ten out of the 13 genes were also light-upregulated in both the leaf and seedling shoot and had significantly correlated expression profiles with TaNF-YC11. The potential target genes for TaNF-YC11 include subunit members from all four thylakoid membrane-bound complexes required for the conversion of solar energy into chemical energy and rate-limiting enzymes in the Calvin cycle. These data indicate that TaNF-YC11 is potentially involved in regulation of photosynthesis-related genes.
Agid:
2260901