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HvNax3—a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum)

Author:
Shavrukov, Yuri, Gupta, Narendra K., Miyazaki, Junji, Baho, Manahil N., Chalmers, Kenneth J., Tester, Mark, Langridge, Peter, Collins, Nicholas C.
Source:
Functional & integrative genomics 2010 v.10 no.2 pp. 277-291
ISSN:
1438-793X
Subject:
Brachypodium, Hordeum vulgare subsp. spontaneum, barley, cultivars, genes, hydroponics, introgression, loci, proteins, quantitative trait loci, rice, salt tolerance, shoots, sodium, sodium chloride
Abstract:
Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na⁺) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection of the cultivated barley (H. vulgare ssp. vulgare) cultivar Barque (Barque-73, a moderate Na⁺ excluder) attributed the control of the Na⁺ exclusion trait from CPI-71284-48 to a single locus on the short arm of chromosome 7H, which was named HvNax3. The locus reduced shoot Na⁺ accumulation by 10–25% in plants grown in 150 mM NaCl. Markers generated using colinearity with rice and Brachypodium, together with the analysis of introgression lines and F₂ and F₃ families, enabled HvNax3 to be mapped to a 1.3-cM interval. Genes from the corresponding rice and Brachypodium intervals encode 16 different classes of proteins and include several plausible candidates for HvNax3. The potential of HvNax3 to provide a useful trait contributing to salinity tolerance in cultivated barley is discussed.
Agid:
2264798