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MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor

Author:
Zhu, Li-Hua, Liu, Tao, Tang, Hua, Tian, Rui-Qing, Su, Chang, Liu, Min, Li, Xin
Source:
FEBS journal 2010 v.277 no.18 pp. 3726-3734
ISSN:
1742-464X
Subject:
adenocarcinoma, cell proliferation, fluorescence, gene expression, genes, hepatoma, interleukin-6, messenger RNA, microRNA, neoplasm cells, non-coding RNA, stomach neoplasms, tissues
Abstract:
MicroRNAs are an evolutionarily conserved class of endogenous noncoding RNAs that modulate gene expression at the post-transcriptional level. Recently, microRNA-23a (miR-23a) has been found to function as a growth-promoting and antiapoptotic factor in hepatocellular carcinoma cells. Our previous study showed that miR-23a was significantly upregulated in gastric adenocarcinoma tissues. In this study, we found that miR-23a promoted the proliferative potential of gastric adenocarcinoma cell line MGC803. We also identified IL6R as a direct target gene for miR-23a using a fluorescent reporter assay. The mRNA and protein levels of IL6R were both inversely correlated with the miR-23a expression level. Our results demonstrate that miR-23a can target IL6R and promote the growth activity of gastric adenocarcinoma cells in vitro. The downregulation of IL6R by miR-23a may explain why the suppression of miR-23a can inhibit gastric cancer cell proliferation.
Agid:
2274982