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New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA

Author:
Kempenaers, Morgane, Roovers, Martine, Oudjama, Yamina, Tkaczuk, Karolina L., Bujnicki, Janusz M., Droogmans, Louis
Source:
Nucleic acids research 2010 v.38 no.19 pp. 6533-6543
ISSN:
0305-1048
Subject:
Sulfolobus, Thermococcus kodakarensis, adenosine, chemical elements, evolution, guanosine, methyltransferases, transfer RNA, yeasts
Abstract:
Two archaeal tRNA methyltransferases belonging to the SPOUT superfamily and displaying unexpected activities are identified. These enzymes are orthologous to the yeast Trm10p methyltransferase, which catalyses the formation of 1-methylguanosine at position 9 of tRNA. In contrast, the Trm10p orthologue from the crenarchaeon Sulfolobus acidocaldarius forms 1-methyladenosine at the same position. Even more surprisingly, the Trm10p orthologue from the euryarchaeon Thermococcus kodakaraensis methylates the N¹-atom of either adenosine or guanosine at position 9 in different tRNAs. This is to our knowledge the first example of a tRNA methyltransferase with a broadened nucleoside recognition capability. The evolution of tRNA methyltransferases methylating the N¹ atom of a purine residue is discussed.
Agid:
2282717