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Magnetically recyclable, antimicrobial, and catalytically enhanced polymer-assisted “green” nanosystem-immobilized Aspergillus niger amyloglucosidase

Konwarh, Rocktotpal, Kalita, Dipankar, Mahanta, Charulata, Mandal, Manabendra, Karak, Niranjan
Applied microbiology and biotechnology 2010 v.87 no.6 pp. 1983-1992
Aspergillus niger, Mesua ferrea, ambient temperature, biocatalysts, detergents, ethylene glycol, immobilized enzymes, iron oxides, leaf extracts, mixing, nanoparticles, nanosilver, polyethylene glycol, recycling, saccharification, starch, washing
The present work reports the integration of polymer matrix-supported nanomaterial and enzyme biotechnology for development of industrially feasible biocatalysts. Aqueous leaf extract of Mesua ferrea L. was used to prepare silver nanoparticles distributed within a narrow size range (1-12 nm). In situ oxidative technique was used to obtain poly(ethylene glycol)-supported iron oxide nanoparticles (3-5 nm). Sonication-mediated mixing of above nanoparticles generated the immobilization system comprising of polymer-supported silver-iron oxide nanoparticles (20-30 nm). A commercially important enzyme, Aspergillus niger amyloglucosidase was coupled onto the immobilization system through sonication. The immobilization enzyme registered a multi-fold increment in the specific activity (807 U/mg) over the free counterpart (69 U/mg). Considerable initial activity of the immobilized enzyme was retained even after storing the system at room temperature as well as post-repeated magnetic recycling. Evaluation of the commendable starch saccharification rate, washing performance synergy with a panel of commercial detergents, and antibacterial potency strongly forwards the immobilized enzyme as a multi-functional industrially feasible system.