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Magnetically recyclable, antimicrobial, and catalytically enhanced polymer-assisted “green” nanosystem-immobilized Aspergillus niger amyloglucosidase
- Konwarh, Rocktotpal, Kalita, Dipankar, Mahanta, Charulata, Mandal, Manabendra, Karak, Niranjan
- Applied microbiology and biotechnology 2010 v.87 no.6 pp. 1983-1992
- Aspergillus niger, Mesua ferrea, ambient temperature, biocatalysts, detergents, ethylene glycol, immobilized enzymes, iron oxides, leaf extracts, mixing, nanoparticles, nanosilver, polyethylene glycol, recycling, saccharification, starch, washing
- The present work reports the integration of polymer matrix-supported nanomaterial and enzyme biotechnology for development of industrially feasible biocatalysts. Aqueous leaf extract of Mesua ferrea L. was used to prepare silver nanoparticles distributed within a narrow size range (1-12 nm). In situ oxidative technique was used to obtain poly(ethylene glycol)-supported iron oxide nanoparticles (3-5 nm). Sonication-mediated mixing of above nanoparticles generated the immobilization system comprising of polymer-supported silver-iron oxide nanoparticles (20-30 nm). A commercially important enzyme, Aspergillus niger amyloglucosidase was coupled onto the immobilization system through sonication. The immobilization enzyme registered a multi-fold increment in the specific activity (807 U/mg) over the free counterpart (69 U/mg). Considerable initial activity of the immobilized enzyme was retained even after storing the system at room temperature as well as post-repeated magnetic recycling. Evaluation of the commendable starch saccharification rate, washing performance synergy with a panel of commercial detergents, and antibacterial potency strongly forwards the immobilized enzyme as a multi-functional industrially feasible system.