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LC Determination of trans-3,5,3′,4′,5′-Pentamethoxystilbene in Rat Plasma

Author:
Lin, Hai-Shu, Tringali, Corrado, Spatafora, Carmela, Choo, Qiu-Yi, Ho, Paul C.
Source:
Chromatographia 2010 v.72 no.9-10 pp. 827-832
ISSN:
0009-5893
Subject:
acetonitrile, chromatography, drugs, pharmacokinetics, rats
Abstract:
A simple LC method has been developed and validated to determine trans-3,5,3′,4′,5′-pentamethoxystilbene (PMS) in rat plasma. Chromatographic separation was achieved through gradient delivery of acetonitrile and water (1.5 mL min⁻¹) at 50 °C. PMS was quantified using UV detection at 320 nm. The standard curve ranged from 15 to 1,500 ng mL⁻¹. The intra- and inter-day precisions, in terms of CV, were all less than 10% while the inter-day and intra-day bias ranged from −6.8 to 8.3%. The plasma PMS levels were monitored in Sprague-Dawley rats after drug administration. This simple LC method appears to be useful in the pharmacokinetic investigation of PMS.
Agid:
2311510