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Two enzymatic sources of nitric oxide in different organs of apple plant

Author:
Gao, H. J., Yang, H. Q., Wang, J. Y., Wang, J. X.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 789-792
ISSN:
0006-3134
Subject:
Malus domestica, NAD (coenzyme), apples, developmental stages, leaves, mitochondria, nitric oxide, nitric oxide synthase, nitrites, roots, stems, trees
Abstract:
Nitric oxide production, nitric oxide synthase (NOS) and mitochondrial nitrite-reducing activities in roots, leaves and stems of different developmental stages were investigated, using potted 3-year-old apple (Malus domestica Borkh.) trees. The arginine-dependent NOS activity is sensitive to NOS inhibitor L-NAME and aminoguanidine (AG), with L-NAME being more effective than AG. Endogenous NO production, NOS and mitochondrial nitrite-reducing activities are predominately presented in young leaves and especially in young white roots and young stems. Root and stem mitochondria can reduce nitrite to nitric oxide at the expense of NADH, however, this mitochondrial nitrite-reducing activity is absent in leaves.
Agid:
2311687