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Transcriptomic changes in the root of oil palm (Elaeis guineensis Jacq.) upon inoculation with Bacillus sphaericus UPMB10

Lim, Kok-Ang, Shamsuddin, Zulkifli Hj, Ho, Chai-Ling
Tree genetics & genomes 2010 v.6 no.5 pp. 793-800
Bacillus sphaericus, Elaeis guineensis, abiotic stress, complementary DNA, gene expression, genes, inoculum, microarray technology, morphogenesis, nitrates, nutrition, plant growth, plant growth-promoting rhizobacteria, plantlets, protein synthesis, rhizosphere bacteria, ribosomal proteins, roots, transcriptomics
Plant growth-promoting bacteria (PGPB) promote plant growth through a combination of mechanisms which affect mineral nutrition and root development. In this study, we profiled the transcripts in oil palm roots inoculated with a PGPB, Bacillus sphaericus UPMB10, upon the establishment of a stable inoculum on the root surface by cDNA microarray analysis. A total of 70 from more than 3,700 transcripts examined were found to be significantly upregulated in the roots of oil palm inoculated with B. sphaericus UPMB10 compared to those from un-inoculated oil palm roots. Majority of these genes were related to protein synthesis and processing, stress, primary metabolism, and membrane transport. Many ribosomal proteins were upregulated in the roots of B. sphaericus-inoculated oil palm plantlets. In addition, B. sphaericus UPMB10 were able to upregulate the expression of a gene encoding low affinity nitrate transporter, independent of root morphogenesis. These rhizosphere bacteria were also demonstrated to induce stress-related proteins that may lead to tolerance to biotic and abiotic stresses.