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Asian-common strains of ‘Candidatus Liberibacter asiaticus' are distributed in Northeast India, Papua New Guinea and Timor-Leste

Miyata, Shin-ichi, Kato, Hiroshi, Davis, Richard, Smith, Malcolm W., Weinert, Matthew, Iwanami, Toru
Journal of general plant pathology 2011 v.77 no.1 pp. 43-47
Candidatus Liberibacter asiaticus, genes, greening disease, leaves, nucleotide sequences, phylogeny, polymerase chain reaction, ribosomal DNA, single nucleotide polymorphism, East Timor, India, Japan, Papua New Guinea, Taiwan, Vietnam
‘Candidatus Liberibacter asiaticus' is the most widespread of the three species of ‘Ca. Liberibacter' that cause citrus greening disease (huanglongbing). To ascertain the phylogenetic relationships among Indian isolates that have higher diversity in the 16S rDNA than Asian isolates of this species, we collected symptomatic leaves from Northeast India, Papua New Guinea and Timor-Leste (East Timor) and detected ‘Ca. L. asiaticus' by PCR using primers specific for nusG-rplK genes and 16S rDNA. Phylogenetic analysis with 16S rDNA sequences and single nucleotide polymorphisms of the omp gene region revealed that the Northeast Indian isolates were genetically closer to Asian-common isolates from Japan, Taiwan, and Vietnam than to Indian isolates reported previously. Thus, the Asian-common strains of ‘Ca. L. asiaticus' are apparently also present in Northeast India.