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Purification of C-Phycocyanin from Spirulina platensis by Single-Step Ion-Exchange Chromatography
- Liao, Xiaoxia, Zhang, Bochao, Wang, Xiaoqin, Yan, Huidan, Zhang, Xuewu
- Chromatographia 2011 v.73 no.3-4 pp. 291-296
- Spirulina platensis, activated carbon, adsorption, algae, chitosan, electrophoresis, fluorescence emission spectroscopy, gels, ion exchange chromatography, phycocyanin, potassium phosphates, sodium dodecyl sulfate, spectral analysis, ultraviolet-visible spectroscopy
- C-Phycocyanin is a natural blue pigment with many commercial applications in foods, cosmetics, and medicines. In this paper we describe the extraction and purification of C-phycocyanin from the cyanobacterium Spirulina platensis. The procedure is based on adsorption of impurities with chitosan and activated charcoal then one-step ion-exchange chromatography. The dry algal powder was soaked in potassium phosphate buffer for 2 h to furnish crude phycocyanin extract of purity 0.93. The crude extract was then treated with chitosan and activated charcoal for 5 min, which increased the purity to 2.78. After chromatography on DEAE Sephadex A-25, the purity of phycocyanin was improved to 4.3. The identity of the purified phycocyanin was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis and by spectroscopic measurements (UV-visible spectrophotometry and spectrofluorimetry). Compared with conventional methods, this method was simple, inexpensive, and time-saving.