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Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacillus cereus isolate from Malaysian rainforest soil

Author:
Chan, Kok-Gan, Wong, Cheng-Siang, Yin, Wai-Fong, Sam, Choon-Kook, Koh, Chong-Lek
Source:
Antonie van Leeuwenhoek 2010 v.98 no.3 pp. 299-305
ISSN:
0003-6072
Subject:
Bacillus cereus, bacteria, enzyme inactivation, homoserine, lactones, liquid chromatography, nucleotide sequences, rain forests, ribosomal DNA, sequence analysis, soil
Abstract:
A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. KM1S was clustered closely to Bacillus cereus by 16S ribosomal DNA sequence analysis. It degraded N-3-oxo-hexanoyl homoserine lactone and N-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 μg AHL h⁻¹ per 10⁹ CFU/ml, respectively, as determined by the Rapid Resolution Liquid Chromatography. The aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of N-acylhomoserine lactones, of KM1S was amplified and cloned. Sequence analysis indicated the presence of the motif ₁₀₆HXDH-59 amino acids-H₁₆₉-21 amino acids-D₁₉₁ for N-acylhomoserine lactone lactonases.
Agid:
2317925