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Predicting promoter activities of primary human DNA sequences

Author:
Irie, Takuma, Park, Sung-Joon, Yamashita, Riu, Seki, Masahide, Yada, Tetsushi, Sugano, Sumio, Nakai, Kenta, Suzuki, Yutaka
Source:
Nucleic acids research 2011 v.39 no.11 pp. e75
ISSN:
0305-1048
Subject:
DNA, DNA-directed RNA polymerase, computer software, correlation, genome, humans, luciferase, models, nucleosomes, nucleotide sequences, prediction, regression analysis, sequence analysis, transcription (genetics)
Abstract:
We developed a computer program that can predict the intrinsic promoter activities of primary human DNA sequences. We observed promoter activity using a quantitative luciferase assay and generated a prediction model using multiple linear regression. Our program achieved a prediction accuracy correlation coefficient of 0.87 between the predicted and observed promoter activities. We evaluated the prediction accuracy of the program using massive sequencing analysis of transcriptional start sites in vivo. We found that it is still difficult to predict transcript levels in a strictly quantitative manner in vivo; however, it was possible to select active promoters in a given cell from the other silent promoters. Using this program, we analyzed the transcriptional landscape of the entire human genome. We demonstrate that many human genomic regions have potential promoter activity, and the expression of some previously uncharacterized putatively non-protein-coding transcripts can be explained by our prediction model. Furthermore, we found that nucleosomes occasionally formed open chromatin structures with RNA polymerase II recruitment where the program predicted significant promoter activities, although no transcripts were observed.
Agid:
2322530