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Loop-mediated isothermal amplification for the rapid detection of Salmonella

Author:
Hara-Kudo, Yukiko, Yoshino, Manabu, Kojima, Tadashi, Ikedo, Masanari
Source:
FEMS microbiology letters 2005 v.253 no.1 pp. 155-161
ISSN:
0378-1097
Subject:
Salmonella enterica subsp. arizonae, Salmonella enterica subsp. enterica, eggs, fluorescence, loop-mediated isothermal amplification, polymerase chain reaction, serotypes, turbidity
Abstract:
Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay was found to be >2.2 cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay. S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8 cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for Salmonella.
Agid:
2328503