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Comparison and characterization of polysaccharides from natural and cultured Cordyceps using saccharide mapping

Author:
Guan, Jia, Zhao, Jing, Feng, Kun, Hu, De-Jun, Li, Shao-Ping
Source:
Analytical and bioanalytical chemistry 2011 v.399 no.10 pp. 3465-3474
ISSN:
1618-2642
Subject:
Cordyceps, glycosidic linkages, high performance liquid chromatography, hydrolysates, physicochemical properties, polygalacturonase, polysaccharides, quality control
Abstract:
Comparison and characterization of polysaccharides from natural and cultured Cordyceps on the basis of their chemical characteristics such as glycosidic linkages were performed for the first time using saccharide mapping. The results showed that polysaccharides from most of the natural and cultured Cordyceps had similar responses to enzymatic digestion. These polysaccharides mainly contained (1→4)-β-D-glucosidic linkages, and (1→4)-α-glucosidic, (1→6)-α-glucosidic, 1,4-β-D-mannosidic, as well as (1→4)-α-D-galactosiduronic linkages also existed in some polysaccharides. Especially, natural and cultured Cordyceps polysaccharides could be discriminated on the basis of high performance liquid chromatography profiles of pectinase hydrolysates, which is helpful to control the quality of polysaccharides from Cordyceps. [graphic removed]
Agid:
2329783