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The Kinetics of Renaturation of 5-S RNA from Escherichia coli in the Presence of Mg²⁺ Ions

Author:
Richards, Edward G., Lecanidou, Rena, Geroch, Mary E.
Source:
European journal of biochemistry 1973 v.34 no.2 pp. 262-267
ISSN:
0014-2956
Subject:
Escherichia coli, RNA, absorbance, activation energy, electrophoresis, ions, magnesium, temperature
Abstract:
The kinetics of renaturation of denatured 5-S RNA from Escherichia coli have been studied by estimating the composition of reaction mixtures from absorbance profiles of stained zones containing the two forms separated by polyacrylamide-gel electrophoresis. The kinetics are first order and proceed to completion irrespective of temperature or Mg²⁺ ion concentration, but they depend strongly on temperature. Linear Arrhenius plots yield an activation energy of 65 kcal/mol RNA. It is suggested that about nine base pairs of the denatured form dissociate and about the same number reform in a different configuration in the renatured conformer.
Agid:
2331503