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Reverse Homogeneous Liquid-Liquid Extraction as a Miniaturized Method for Extraction of Aflatoxins from Pistachio and Wheat and LC Post-column Derivatization-Fluorescence Detection

Author:
Hassan, Jalal, Habibi, Shahoo
Source:
Chromatographia 2011 v.73 no.9-10 pp. 1005-1008
ISSN:
0009-5893
Subject:
aflatoxins, chloroform, detection limit, high performance liquid chromatography, liquid-liquid extraction, pistachios, potassium bromide, quantitative analysis, wheat
Abstract:
A rapid and simple quantitative method for the simultaneous determination of the four aflatoxins (AFs) B1, B2, G1 and G2 was developed using reverse homogeneous liquid-liquid extraction (RHLEE) and HPLC post-column derivatization-fluorescence (HPLC-FL) detection. The method based on the rapid extraction of AFs from a methanolic sample into chloroform after addition of water containing KBr, with a method we called reverse homogeneous liquid-liquid extraction. Recoveries were in the range of 88-125% and the limits of detection were between 0.001-0.042 ng g⁻¹ for different AFs. Wheat and pistachio were chosen for the analysis of real samples and the method was also successfully applied to a FAPAS® TEST MATERIAL (T04151).
Agid:
2335054