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LC-MS/MS Assay for Quantification of a Novel Antidiabetic Flavone Derivative S002-857 in Rat Plasma, Urine and Feces: Application in Pharmacokinetic Studies

Gautam, Nagsen, Kushwaha, Hari Narayan, Kumar, Hemant, Pratap, Ram, Singh, Shio Kumar
Chromatographia 2011 v.73 no.9-10 pp. 905-912
acetates, acetonitrile, ambient temperature, chromatography, drugs, ethyl ether, excretion, feces, freeze-thaw cycles, liquid-liquid extraction, males, pH, pharmacokinetics, rats, sampling, urine
This study aimed to develop an LC-MS/MS assay for the quantitation of S002-857 in plasma, urine and feces to evaluate the pharmacokinetic parameters and elimination pathway for further development of S002-857. A sensitive and selective LC-MS/MS method was developed and validated for estimating a novel antidiabetic flavone derivative, S002-857, synthesized by Central Drug Research Institute, CSIR, Lucknow, India. Plasma and urine samples were prepared by two-step liquid-liquid extraction with diethyl ether and feces by protein precipitation using acetonitrile. The analyte was chromatographed on a cyano column with methanol-ammonium acetate buffer (pH 4.6, 10 mM; 90:10, v/v). The calibration curves were linear over a range of 0.78-400 ng mL⁻¹ for plasma and 1.56-400 ng mL⁻¹ for urine and feces samples. The accuracy and precision were <15% for plasma, urine and feces samples. The recoveries from spiked plasma, urine and feces samples were >85%. S002-857 was stable in plasma for 8 h at ambient temperature, 30 days at −60 °C and after three freeze-thaw cycles. The assay was successfully applied to determine the pharmacokinetic parameters and excretion profile in male rats.