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TEFM (c17orf42) is necessary for transcription of human mtDNA

Author:
Minczuk, Michal, He, Jiuya, Duch, Anna M., Ettema, Thijs J., Chlebowski, Aleksander, Dzionek, Karol, Nijtmans, Leo G.J., Huynen, Martijn A., Holt, Ian J.
Source:
Nucleic acids research 2011 v.39 no.10 pp. 4284-4299
ISSN:
0305-1048
Subject:
DEAD-box RNA helicases, DNA-directed RNA polymerase, RNA interference, cultured cells, humans, mitochondria, mitochondrial DNA, mitochondrial RNA, mutants, ribonucleases, transcription (genetics)
Abstract:
Here we show that c17orf42, hereafter TEFM (transcription elongation factor of mitochondria), makes a critical contribution to mitochondrial transcription. Inactivation of TEFM in cells by RNA interference results in respiratory incompetence owing to decreased levels of H- and L-strand promoter-distal mitochondrial transcripts. Affinity purification of TEFM from human mitochondria yielded a complex comprising mitochondrial transcripts, mitochondrial RNA polymerase (POLRMT), pentatricopeptide repeat domain 3 protein (PTCD3), and a putative DEAD-box RNA helicase, DHX30. After RNase treatment only POLRMT remained associated with TEFM, and in human cultured cells TEFM formed foci coincident with newly synthesized mitochondrial RNA. Based on deletion mutants, TEFM interacts with the catalytic region of POLRMT, and in vitro TEFM enhanced POLRMT processivity on ss- and dsDNA templates. TEFM contains two HhH motifs and a Ribonuclease H fold, similar to the nuclear transcription elongation regulator Spt6. These findings lead us to propose that TEFM is a mitochondrial transcription elongation factor.
Agid:
2342443