PubAg

Main content area

Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry

Author:
Chi, An, Huttenhower, Curtis, Geer, Lewis Y., Coon, Joshua J., Syka, John E.P., Bai, Dina L., Shabanowitz, Jeffrey, Burke, Daniel J., Troyanskaya, Olga G., Hunt, Donald F.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.7 pp. 2193-2198
ISSN:
0027-8424
Subject:
Saccharomyces cerevisiae, yeasts, fungal proteins, protein phosphorylation, mass spectrometry, proteome, phosphoproteins
Abstract:
We present a strategy for the analysis of the yeast phosphoproteome that uses endo-Lys C as the proteolytic enzyme, immobilized metal affinity chromatography for phosphopeptide enrichment, a 90-min nanoflow-HPLC/electrospray-ionization MS/MS experiment for phosphopeptide fractionation and detection, gas phase ion/ion chemistry, electron transfer dissociation for peptide fragmentation, and the Open Mass Spectrometry Search Algorithm for phosphoprotein identification and assignment of phosphorylation sites. From a 30-μg ([almost equal to]600 pmol) sample of total yeast protein, we identify 1,252 phosphorylation sites on 629 proteins. Identified phosphoproteins have expression levels that range from <50 to 1,200,000 copies per cell and are encoded by genes involved in a wide variety of cellular processes. We identify a consensus site that likely represents a motif for one or more uncharacterized kinases and show that yeast kinases, themselves, contain a disproportionately large number of phosphorylation sites. Detection of a pHis containing peptide from the yeast protein, Cdc10, suggests an unexpected role for histidine phosphorylation in septin biology. From diverse functional genomics data, we show that phosphoproteins have a higher number of interactions than an average protein and interact with each other more than with a random protein. They are also likely to be conserved across large evolutionary distances.
Agid:
2351579