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A polyphosphate kinase 1 (ppk1) mutant of Pseudomonas aeruginosa exhibits multiple ultrastructural and functional defects

Author:
Fraley, Cresson D., Rashid, M. Harunur, Lee, Sam S.K., Gottschalk, Rebecca, Harrison, Janine, Wood, Pauline J., Brown, Michael R.W., Kornberg, Arthur
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.9 pp. 3526-3531
ISSN:
0027-8424
Subject:
Escherichia coli, Pseudomonas aeruginosa, bacteria, biofilm, carbenicillin, mutants, quorum sensing, virulence
Abstract:
Pseudomonas aeruginosa, of medical, environmental, and industrial importance, depends on inorganic polyphosphate (poly P) for a wide range of functions, especially survival. Mutants of PAO1 lacking poly P kinase 1, PPK1, the enzyme responsible for most poly P synthesis in Escherichia coli and other bacteria, are defective in motility, quorum sensing, biofilm formation, and virulence. We describe here multiple defects in the ppk1 mutant PAOM5, including a striking compaction of the nucleoid, distortion of the cell envelope, lack of planktonic motility and exopolymer production, and susceptibility to the β-lactam antibiotic carbenicillin as well as desiccation. We propose that P. aeruginosa with reduced poly P levels undergoes ultrastructural changes that contribute to profound deficiencies in cellular functions.
Agid:
2351804