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The RNA of RNase MRP is required for normal processing of ribosomal RNA

Author:
Chu, S., Archer, R.H., Zengel, J.M., Lindahl, L.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.2 pp. 659-663
ISSN:
0027-8424
Subject:
ribosomal RNA, structural genes, chemical reactions, ribosomal DNA, ribonucleoproteins, Saccharomyces cerevisiae, ribonucleases
Abstract:
We have isolated clones which complement the temperature sensitivity and abnormal rRNA processing pattern of the rrp2-2 mutant of Saccharomyces cerevisiae we previously described. DNA sequencing and restriction analysis demonstrated that all clones contain the NME1 gene encoding the RNA of the ribonucleoprotein particle RNase MRP. Deletion analysis showed that the NME1 gene is responsible for the complementation of the rrp2-2 phenotype. A single base change was identified in the nme1 gene in the rrp2 mutant, confirming that the RRP2 and NME1 genes are identical. Our experiments therefore indicate that RNase MRP, in addition to its previously reported role in formation of RNA primers for mitochondrial DNA replication [Clayton, D. A. (1991) Trends Biochem. Sci. 16, 107-111], is involved in rRNA processing.
Agid:
2355013