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Temporal phosphorylation of the Drosophila period protein

Author:
Edery, I., Zwiebel, L.J., Dembinska, M.E., Rosbash, M.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.6 pp. 2260-2264
ISSN:
0027-8424
Subject:
Drosophila melanogaster, circadian rhythm, animal proteins, phosphorylation, structural genes
Abstract:
The period gene (per) is required for Drosophila melanogaster to manifest circadian (approximately 24 hr) rhythms. We report here that per protein (PER) undergoes daily oscillations in apparent molecular mass as well as abundance. The mobility changes are largely or exclusively due to multiple phosphorylation events. The temporal profile of the classic short-period form of PER (PERS) is altered in a manner consistent with the mutant strain's behavioral phenotype. As changes in abundance and phosphorylation persist under constant environmental conditions, they reflect or contribute to a free-running rhythm. We suggest that the phosphorylation status of PER is an important determinant in the Drosophila clock's time-keeping mechanism.
Agid:
2355049