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Nuclear transformation of Volvox carteri

Author:
Schiedlmeier, B., Schmitt, R., Muller, W., Kirk, M.M., Gruber, H., Mages, W., Kirk, D.L.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.11 pp. 5080-5084
ISSN:
0027-8424
Subject:
Chlorophycota, genetic transformation, direct DNA uptake, reporter genes, structural genes, nitrate reductase, genetic recombination
Abstract:
Stable nuclear transformation of Volvox carteri was achieved using the cloned V. carteri nitA+ gene (which encodes nitrate reductase) to complement a nitA- mutation. Following bombardment of mutant cells with plasmid-coated gold particles, putative transformants able to utilize nitrate as a nitrogen source were recovered with an efficiency of approximately 2.5 X 10(-5). DNA analysis indicated that the plasmid integrated into the genome, often in multiple copies, at sites other than the nitA locus. Cotransformants were recovered with a frequency of 40-80% when cells were cobombarded with a selected and an unselected marker. Thus, V. carteri becomes one of the simplest multicellular organisms that is accessible to detailed molecular studies of genes regulating cellular differentiation and morphogenesis.
Agid:
2355180