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Activity of the Chlamydomonas chloroplast rbcL gene promoter is enhanced by a remote sequence element
- Klein, U., Salvador, M.L., Bogorad, L.
- Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.23 pp. 10819-10823
- Chlamydomonas reinhardtii, structural genes, ribulose-bisphosphate carboxylase, recombinant DNA, reporter genes, beta-glucuronidase, gene expression, promoter regions, transcription (genetics)
- The chloroplast gene rbcL encodes the large subunit of ribulose bisphosphate carboxylase. In Chlamydomonas reinhardtii, this gene is transcribed more actively than any other protein-encoding chloroplast gene studied to date. To delineate the rbcL gene promoter, chimeric reporter genes containing fragments of the 5' region of the rbcL gene fused to the coding sequence of the bacterial uidA gene. encoding beta-glucuronidase, were stably introduced into the chloroplast genome of Chlamydomonas by microprojectile bombardment. The relative transcription rates of endogenous and introduced genes were determined in transgenic cell lines in vivo. The basic rbcL promoter is located within the region of the gene extending from positions -18 to +63, taking position +1 as the site of initiation of transcription. A chimeric reporter gene containing only the basic promoter is transcribed only 1-15% as actively as the endogenous rbcL gene, depending on the conditions under which cells are grown and tested. However, a chimeric gene containing rbcL sequences extending to position +170 or beyond is transcribed at about the same rate as the endogenous gene. Deletion of the sequence between positions +170 and +126, well within the protein-encoding region, reduces the rate of transcription to that of reporter genes with the basic promoter alone.