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Cloning, expression, and function of TFC5, the gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIB
- Kassavetis, G.A., Nguyen, S.T., Kobayashi, R., Kumar, A., Geiduschek, E.P., Pisano, M.
- Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.21 pp. 9786-9790
- Saccharomyces cerevisiae, structural genes, DNA-binding proteins, DNA-directed RNA polymerase, nucleotide sequences, amino acid sequences, DNA, gene expression, transcription (genetics), binding sites
- TFC5, the unique and essential gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor (TF)IIIB has been cloned. It encodes a 594-amino acid protein (67,688 Da). Escherichia coli-produced B" has been used to reconstitute entirely recombinant TFIIIB that is fully functional for TFIIIC-directed, as well as TATA box-dependent, DNA binding and transcription. The DNase I footprints of entirely recombinant TFIIIB, composed of B", the 67-kDa Brf, and TATA box-binding protein, and TFIIIB reconstituted with natural B" are indistinguishable. A truncated form of B" lacking 39 N-terminal and 107 C-terminal amino acids is also functional for transcription.