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Intron of the mouse Hoxa-7 gene contains conserved homeodomain binding sites that can function as an enhancer element in Drosophila

Author:
Haerry, T.E., Gehring, W.J.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1996 v.93 no.24 pp. 13884-13889
ISSN:
0027-8424
Subject:
Drosophila melanogaster, mice, introns, gene transfer, genetic transformation, transgenic animals, homeotic genes, DNA-binding proteins, gene expression, reporter genes, beta-galactosidase, recombinant DNA, binding sites
Abstract:
The 5' flanking sequences and the intron of the mouse Hoxa-7 gene were searched for regulatory elements that can function in Drosophila. Only the intron is able to activate a lacZ fusion gene in various tissues of Drosophila embryos. This enhancer function requires a cluster of three homeodomain binding sites (HB1-element) that are also found in the introns of other Hox genes as well as in a putative autoregulatory element of Ultrabithorax (Ubx), the Drosophila homolog of Hoxa-7. If a single binding site in the autoregulatory element of fushi tarazu (ftz) is replaced by the HB1-element Hoxa-7, the expression pattern is altered and newly controlled by the homeotic gene caudal (cad). These data suggest that HB1 is a potential target for different homeodomain proteins of both vertebrates and invertebrates.
Agid:
2356314