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Jasmonate is essential for insect defense in Arabidopsis

Author:
McConn, M., Creelman, R.A., Bell, E., Mullet, J.E., Browse, J.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.10 pp. 5473-5477
ISSN:
0027-8424
Subject:
Arabidopsis thaliana, Sciaridae, insect pests, larvae, defense mechanisms, jasmonic acid, mutants, linolenic acid, gene expression, messenger RNA, phenylalanine ammonia-lyase, acid phosphatase, glutathione transferase, mechanical damage, methyl jasmonate
Abstract:
The signaling pathways that allow plants to mount defenses against chewing insects are known to be complex. To investigate the role of jasmonate in wound signaling in Arabidopsis and to test whether parallel or redundant pathways exist for insect defense, we have studied a mutant (fad3-2fad7-2fad8) that is deficient in the jasmonate precursor linolenic acid. Mutant plants contained negligible levels of jasmonate and showed extremely high mortality (approximately 80%) from attack by larvae of a common saprophagous fungal gnat, Bradysia impatiens (Diptera: Sciaridae), even though neighboring wild-type plants were largely unaffected. Application of exogenous methyl jasmonate substantially protected the mutant plants and reduced mortality to approximately 12%. These experiments precisely define the role of jasmonate as being essential for the induction of biologically effective defense in this plant-insect interaction. The transcripts of three wound-responsive genes were shown not to he induced by wounding of mutant plants but the same transcripts could be induced by application of methyl jasmonate. By contrast, measurements of transcript levels for a gene encoding glutathione S-transferase demonstrated that wound induction of this gene is independent of jasmonate synthesis. These results indicate that the mutant will be a good genetic model for testing the practical effectiveness of candidate defense genes.
Agid:
2356500