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Epidermal muscle attachment site-specific target gene expression and interference with myotube guidance in response to ectopic stripe expression in the developing Drosophila epidermis

Author:
Vorbruggen, G., Jackle, H.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.16 pp. 8606-8611
ISSN:
0027-8424
Subject:
Drosophila melanogaster, transcription factors, mutants, gene expression, embryogenesis, muscles, integument, transgenic animals, embryo (animal)
Abstract:
The egr-type zinc-finger transcription factor encoded by the Drosophila gene stripe (sr) is expressed in a subset or epidermal cells to which muscles attach during late stages of embryogenesis. We report loss-of-function and gain-of-function experiments indicating that sr activity provides ectodermal cells with properties required for the establishment or a normal muscle pattern during embryogenesis and for the differentiation of tendon-like epidermal muscle attachment sites (EMA). Our results show that sr encodes a transcriptional activator which acts as an autoregulated developmental switch gene. sr activity controls the expression of EMA-specific target genes in cells of ectodermal but not of mesodermal origin. sr-expressing ectodermal cells generate long-range signals that interfere with the spatial orientation of the elongating myotubes.
Agid:
2356583