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Plasmon analyses of Triticum (wheat) and Aegilops: PCR-single-strand conformational polymorphism (PCR-SSCP) analyses of organellar DNAs

Author:
Wang, G.Z., Miyashita, N.T., Tsunewaki, K.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.26 pp. 14570-14577
ISSN:
0027-8424
Subject:
Triticum, Aegilops, chloroplast DNA, mitochondrial DNA, genetic markers, genetic polymorphism, genetic variation, polymerase chain reaction, phylogeny, genetic distance, genome, species differences
Abstract:
To investigate phylogenetic relationships among plasmons in Triticum and Aegilops, PCR-single-strand conformational polymorphism (PCR-SSCP) analyses were made of 14.0-kb chloroplast (ct) and 13.7-kb mitochondrial (mt)DNA regions that were isolated from 46 alloplasmic wheat lines and one euplasmic line. These plasmons represent 31 species of the two genera. The ct and mtDNA regions included 10 and 9 structural genes respectively. A total of 177 bands were detected of which 40.6% were variable. The proportion of variable bands in ctDNA (51.1%) was higher than that of mtDNA (28.9%). The phylogenetic trees of plasmons. derived by two different models indicate a common picture of plasmon divergence in the two genera and suggest three major groups of plasmons (Einkorn Triticum, and Aegilops). Because of uniparental plasmon transmission the maternal parents of all but one polyploid species were identified. Only one Aegilops species, Ae. speltoides, was included in the Triticum group suggesting that this species is the plasmon and B and G genome donor of all polyploid wheats. ctDNA variations were more intimately correlated with vegetative characters, whereas mtDNA variations were more closely correlated with reproductive characters. Plasmon divergence among the diploids of the two genera largely paralleled genome divergence. The relative times of origin of the polyploid species were inferred from genetic distances from their putative maternal parents.
Agid:
2356778