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Calx, a Na-Ca exchanger gene of Drosophila melanogaster

Author:
Schwarz, E.M., Benzer, S.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1997 v.94 no.19 pp. 10249-10254
ISSN:
0027-8424
Subject:
messenger RNA, structural genes, complementary DNA, animal proteins, open reading frames, calcium, ion transport, amino acid sequences, gene expression, repetitive sequences, chromosome mapping, sodium, plasma membrane, Drosophila melanogaster, inorganic ions
Abstract:
We have cloned Calx, a gene that encodes a Na-Ca exchanger of Drosophila melanogaster. Calx encodes two repeated motifs, Calx-alpha and Calx-beta, that overlap domains required for exchanger activity and regulation. Calx has multiple transcripts in adults, including at least one expressed in the retina. The Calx genomic locus comprises greater or equal to 35 kb between the Atp alpha and rudimentary-like genes in chromosomal region 93B. In Xenopus oocytes, microinjected Calx cRNA induces calcium uptake like that of its homolog, the 3Na+-1Ca2+ exchanger of mammalian heart. Implications of Calx-alpha motifs for the mechanism of Na-Ca exchange are discussed.
Agid:
2356811