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Acute carbon dioxide avoidance in Caenorhabditis elegans

Author:
Hallem, Elissa A., Sternberg, Paul W.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.23 pp. 8038-8043
ISSN:
0027-8424
Subject:
Caenorhabditis elegans, adults, animals, avoidance behavior, carbon dioxide, cell respiration, cyclic GMP, insulin, neural networks, neuropeptide Y, nutritional status, physiological state, predators, sensory neurons, signal transduction, transforming growth factor beta
Abstract:
Carbon dioxide is produced as a by-product of cellular respiration by all aerobic organisms and thus serves for many animals as an important indicator of food, mates, and predators. However, whether free-living terrestrial nematodes such as Caenorhabditis elegans respond to CO₂ was unclear. We have demonstrated that adult C. elegans display an acute avoidance response upon exposure to CO₂ that is characterized by the cessation of forward movement and the rapid initiation of backward movement. This response is mediated by a cGMP signaling pathway that includes the cGMP-gated heteromeric channel TAX-2/TAX-4. CO₂ avoidance is modulated by multiple signaling molecules, including the neuropeptide Y receptor NPR-1 and the calcineurin subunits TAX-6 and CNB-1. Nutritional status also modulates CO₂ responsiveness via the insulin and TGFβ signaling pathways. CO₂ response is mediated by a neural circuit that includes the BAG neurons, a pair of sensory neurons of previously unknown function. TAX-2/TAX-4 function in the BAG neurons to mediate acute CO₂ avoidance. Our results demonstrate that C. elegans senses and responds to CO₂ using multiple signaling pathways and a neural network that includes the BAG neurons and that this response is modulated by the physiological state of the worm.
Agid:
2359204